Isolation, Purification, Structural Characterization, and Hypoglycemic Activity of Polysaccharide from Trichosanthes kirilowii Maxim. Seed Shell

In this manuscript, polysaccharide (TMSP) was isolated from Trichosanthes kirilowii Maxim. seed shell. The homogeneous (TMSP-1; molecular weight: 690.239 kDa) purified by column chromatography (DEAE-cellulose-52 and Sephadex G-150). result of monosaccharide composition showed that TMSP-1 consisted D-mannose, D-glucose, D-galactose, glucuronic acid in a molar ratio 2.01 : 1.98 1.87 1. Methylation analysis made up →3)-D-Galp-(1→, D-Glcp-(1→, →4)-D-Manp-(1→, →6)-D-Galp-(1→, →4)-D-Galp-(1→, →4,6)-D-Manp-(1→. microscopic conformation regular flake-like structure inlaid with small holes. α-glucosidase inhibitory rate reached 52.23% when the concentration 8 mg/mL, which confirmed potential hypoglycemic activity vitro. vivo results type 2 diabetes causes significant increase organ index recovered mice ( P < 0.05 ). Furthermore, reversed liver kidney weight indicators abnormality. This research lays foundation for on polysaccharides shell hypoglycemia.